补骨脂素对人牙周膜干细胞增殖、 成骨分化的促进作用及其机制.

Objective To observe the promotional effects of psoralen on proliferation and osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs), and to explore their mechanism. Methods The third-generation hPDLSCs were divided into six groups: the control group and 5, 10, 25, 50 and 100 μmol/L psoralen groups, respectively. HPDLSCs in the 5, 10, 25, 50 and 100 μmol/L psoralen groups were treated with 5, 10, 25, 50 and 100 μmol/L psoralen, and hPDLSCs in the control group were cultured normally. Cell proliferation capacity was measured by CCK-8 assay, alkaline phosphatase (ALP) activity by colorimetric method, and mineralization nodules by alizarin red staining. Another third-generation hPDLSCs were divided into two groups: the control group and 25 μmol/L psoralen group. Cells in the 25 μmol/L psoralen group were treated with 25 μmol/L psoralen, while cells in the control group were cultured normally. Transcription factor 2 (Runx 2) and osteopontin (OPN) mRNA were detected by RT-PCR. Results Compared with the control group, cell proliferation capacity and ALP activity increased in the 5, 10, 25, 50 and 100 μmol/L psoralen groups, and mineralization nodules increased in the 25 μmol/L psoralen group (all P<0. 05) . Compared with the 5 μmol/L psoralen group, cell proliferative capacity increased in the 25, 50 and 100 μmol/L psoralen groups, ALP activity increased in the 10 and 25 μmol/L psoralen groups, and mineralization nodules increased in the 25 μmol/L psoralen group (all P<0. 05) . Compared with the 10 μmol/L psoralen group, cell proliferation capacity increased in the 25, 50 and 100 μmol/L psoralen groups (all P<0. 05) . Compared with the 25 μmol/L group, ALP activity decreased in the 50 and 100 μmol/L psoralen groups (all P<0. 05) . Compared with the control group, relative expression levels of Runx 2 and OPN mRNA increased in the 25 μmol/L psoralen group (all P<0. 05) . Conclusions Psoralen of 5-100 μmol/L promotes the proliferation and osteogenic differentiation of hPDLSCs. It shows a dose-dependent enhancement at 5 to 25 μmol/L, and there is no obvious change at 50 to 100 μmol/L. The mechanism of psoralen promoting the proliferation and osteogenic differentiation of hPDLSCs may be related to the regulation of Runx 2 signaling pathway. [ABSTRACT FROM AUTHOR]