Near-infrared fluorescent indolizine-dicyanomethylene-4H-pyran hybrids for viscosity imaging in living cells.

[Display omitted] • Two organic fluorescent dyes DCM-1 and DCM-2 with near-infrared fluorescence were designed and synthesized. • DCM-1 and DCM-2 have a specific fluorescence response to viscosity. • DCM-1 and DCM-2 can be successfully applied to the imaging of dynamic changes in viscosity in living cells. The development of near-infrared organic fluorescent dyes with tunable emission profiles is highly required in the field of biological sensing and imaging. In this paper, we designed and synthesized two organic fluorescent dyes, DCM-1 and DCM-2, through the hybridization of indolizine and dicyanomethylene-4H-pyran skeleton. These two compounds show near-infrared fluorescence with emission maximum approximately at 640 and 680 nm, respectively. Notably, both DCM-1 and DCM-2 have specific responses to viscosity without being interfered by biological relevant species. Cell experiments demonstrate that DCM-1 and DCM-2 can detect dynamic changes in viscosity within living cells, suggesting their potential applications in chemical biology research. [ABSTRACT FROM AUTHOR]