Isocyanate-mediated immobilization of Candida antarctica lipase B onto resin for efficient 1,3-diacylglycerol bio-synthesis.

In this work, isocyanate-based strategy was developed for efficient immobilization of Candida antarctica lipase B (CALB). Of 41 commercial resins, EP109 was chosen and hydrophobized with hydroxyl-terminated polydimethylsiloxane (HTPDMS) for tert-butyl isocyanate (tBIC)-based CALB immobilization due to high loading amount and specific activity. The tBIC-based strategy exhibited shorter immobilization time, higher loading and activity than adsorption strategy by hydrophobic interaction. When compared with free CALB, EP109-HTPDMS-tBIC@CALB owned improved stability (against acid, alkali, and heat) and storability. Subsequently, EP109-HTPDMS-tBIC@CALB was employed in the bio-synthesis of 1,3-diacylglycerol in a solvent-free system with a yield of 76.31%, which was higher than commercial Novozym 435 (53.93%). EP109-HTPDMS-tBIC@CALB exhibited 76.34% of residual activity after 20 repeat uses. [Display omitted] • EP109 was selected from 41 resins and hydrophobized with HTPDMS, ODA, and APTES, respectively. • Candida antarctica lipase B was immobilized on EP109-HTPDMS via an isocyanate-based strategy. • Isocyanate-based strategy showed shorter time, higher loading and activity than adsorption. • The immobilized lipase catalyzed the synthesis of 1,3-diacylglycerol with a yield of 76.31%; • The immobilized lipase owned great reusability with 76.34% residual activity after 20 cycles. [ABSTRACT FROM AUTHOR]