Deep-rooted winter wheat cover crop promotes subsoil organic carbon storage through improved microbial functional traits.

Emerging microbial trait-based approaches for soil organic carbon (SOC) storage advocate enhancing specific soil microbial functional traits, such as increased carbon use efficiency (CUE) and enzymatic efficiency (EE) and decreased metabolic quotient (qCO 2). These traits facilitate the efficient conversion of crop residues into microbial biomass C (MBC), that is subsequently stabilized as SOC in the soil mineral fraction. However, our current understanding of SOC dynamics in croplands is mainly confined to topsoil or tillage zone, and the field-based evidence for subsoil microbial functional trait dynamics is sparse. To address this knowledge gap, we studied the effect of contrasting long-term agricultural management practices on microbial functional traits favoring SOC accumulation in topsoil and subsoil by conducting a laboratory experiment using 13C labeled glucose addition. Our results show a 19 % increase in C growth (C allocated for microbial biomass growth), 11 % increase in CUE and 14 % increase in EE under no-till (NT) compared to conventional till (CT) in topsoil (0–10 cm) and no difference in these microbial parameters in subsoil (30–40 cm) between NT and CT. Growing a deep-rooted winter wheat (WW) cover crop increased CUE by 28 % in the topsoil and 11 % in the subsoil compared to no-cover treatment. We found separate drivers controlling SOC accumulation in topsoil and subsoil. These drivers are increased particulate organic matter C (POM–C) and C growth , under NT in topsoil and increased C growth over respiration and mineral associated organic matter C (MAOM–C), under WW in subsoil. Overall, our results provide experimental evidence that different microbial functional traits, molded by different long-term conservation practices, dictate SOC storage in agricultural topsoil and subsoil. • Long-term no-tillage improved topsoil microbial carbon use and enzymatic efficiencies. • Deep-rooted cover crop improved subsoil microbial carbon use and enzymatic efficiencies. • Higher particulate organic C and microbial growth increased topsoil C under no-tillage. • Higher particulate organic C and microbial growth with reduced respiration increased subsoil C under winter wheat cover crop. [ABSTRACT FROM AUTHOR]