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Utility of a microRNA panel in diagnosis and prognosis of hepatitis C–associated hepatocellular carcinoma.
- Source :
-
Laboratory Medicine . May2024, Vol. 55 Issue 3, p310-319. 10p. - Publication Year :
- 2024
-
Abstract
- Background Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer and the leading cause of cancer mortality. Various studies have linked dysregulated microRNA expression to liver cancers, but those related to viral hepatitis–related HCC are limited. Methods We investigated the diagnostic and prognostic roles of circulating miR-331-3p, miR-23b-3p, and miR-3194-5p in EDTA-treated blood samples of 50 hepatitis C virus (HCV) HCC patients, 50 HCV cirrhotic patients, and 50 healthy controls using quantitative real-time polymerase chain reaction. Results We found that miR-23b-3p and miR-3194-5p were significantly downregulated, whereas miR-331-3p was upregulated in HCC patients compared with controls. Also, these miRNAs were significantly dysregulated in HCC compared with cirrhotic patients. For the diagnosis of HCC, miR-331-3p and the combined miRNAs panel had the highest area under the curve (AUC), followed by miR-3194-5p. The highest AUC for differentiating metastatic from nonmetastatic patients was shown by miR-331-3p and the combined miRNAs panel, followed by miR-23b-3p. Dysregulation of miRNAs was associated with poor clinicopathological manifestations. Finally, miR-331-3P was found to be an independent risk factor for metastatic lesions in HCC. Conclusion Overall, the assessed miR-331-3p, miR-23b-3p, and miR-3194-5p were significantly associated with poor clinicopathological features of HCC and could be used to discriminate HCV-related HCC patients from cirrhosis and differentiating metastatic from nonmetastatic patients, primarily miR-331-3p along with combined miRNAs. Moreover, miR-331-3p was found to be an independent factor for metastatic lesions. [ABSTRACT FROM AUTHOR]
- Subjects :
- *RISK assessment
*PREDICTIVE tests
*CIRRHOSIS of the liver
*VIRAL hepatitis
*DATA analysis
*RECEIVER operating characteristic curves
*MICRORNA
*POLYMERASE chain reaction
*KRUSKAL-Wallis Test
*DNA
*CHI-squared test
*QUANTITATIVE research
*MANN Whitney U Test
*DESCRIPTIVE statistics
*CLINICAL pathology
*ANALYSIS of variance
*STATISTICS
*HEPATITIS C
*DATA analysis software
*HEPATOCELLULAR carcinoma
*NONPARAMETRIC statistics
*REGRESSION analysis
*SENSITIVITY & specificity (Statistics)
*DISEASE risk factors
*DISEASE complications
Subjects
Details
- Language :
- English
- ISSN :
- 00075027
- Volume :
- 55
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Laboratory Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 177084307
- Full Text :
- https://doi.org/10.1093/labmed/lmad081