Magnetite-driven Bio-Fenton degradation of chloroacetanilide herbicides by a newly isolated hydrogen peroxide producing bacterium Desemzia sp. strain C1.

The efficiency of the Fenton reaction is markedly contingent upon the operational pH related to iron solubility. Therefore, a heterogeneous Fenton reaction has been developed to function at neutral pH. In the present study, the Bio-Fenton reaction was carried out using magnetite (Fe(II)Fe(III) 2 O 4) and H 2 O 2 generated by a newly isolated H 2 O 2 -producing bacterium, Desemzia sp. strain C1 at pH 6.8 to degrade chloroacetanilide herbicides. The optimal conditions for an efficient Bio-Fenton reaction were 10 mM of lactate, 0.5% (w/v) of magnetite, and resting-cells (O.D. 600 = 1) of strain C1. During the Bio-Fenton reaction, 1.8–2.0 mM of H 2 O 2 was generated by strain C1 and promptly consumed by the Fenton reaction with magnetite, maintaining stable pH conditions. Approximately, 40–50% of the herbicides underwent oxidation through non-specific reactions of •OH, leading to dealkylation, dechlorination, and hydroxylation via hydrogen atom abstraction. These findings will contribute to advancing the Bio-Fenton system for non-specific oxidative degradation of diverse organic pollutants under in-situ environmental conditions with bacteria producing high amount of H 2 O 2 and magnetite under a neutral pH condition. [Display omitted] • H 2 O 2 -producing Desemzia sp. strain C1 was isolated from oil-contaminated soil. • Bio-Fenton reaction was performed using magnetite and H 2 O 2 by strain C1 at pH 6.8. • Optimal conditions for an efficient Bio-Fenton reaction were investigated. • Chloroacetanilide herbicides were chosen as a model of environmental pollutants. • The herbicides were degraded via non-specific oxidative reaction of.•OH. [ABSTRACT FROM AUTHOR]