Towards a zero-waste sustainable biorefinery of Codium sp. seaweed: From bioactives application to soil enhancement materials.

In the pursuit of sustainability and circularity, this study aimed to develop a multi-purpose biorefinery pipeline using extracts from the green seaweed Codium sp. and transforming its post-extracted biomass into biochars, emphasizing holistic resource utilization. Five extracts from Codium sp. were evaluated, testing their antioxidant potential, cytotoxicity, photoprotective effects, anti-inflammatory activity, and inhibitory capabilities against enzymes hyaluronidase, collagenase, and elastase. The ethyl acetate extract showed the highest values in the ORAC assay, indicating antioxidant activity. However, no relevant antioxidant activity was observed in the FRAP and DPPH assays for the other extracts. The hydroethanolic extract exhibited the best anti-enzymatic capacity, inhibiting elastase by 88% at 200 μg/mL. It also demonstrated a photoprotective effect and reduced ROS production (30%) induced by UVB exposure. The hydroethanolic and ethanol extracts significantly reduced the concentration of the tumour necrosis factor-alpha (TNF-α) in response to LPS stimulation, indicating anti-inflammatory activity. The hydroethanolic post-extracted biomass was converted into biochars using conventional slow pyrolysis, hydrothermal carbonization (HTC), and acid-mediated carbonization (AMC). The biochars contained 56–75% C and 22–30% O and the one synthesized by HTC had the highest textural development. The biochar obtained by AMC showed better performance in improving soil water retention capacity, suggesting that acid surface chemistry plays a more significant role than porosity. The Codium sp. extracts and biochars produced from the post-extracted biomass have demonstrated potential as a source of valuable bioactive compounds and biomaterials for a circular economy framework and biorefinery pipeline with wide-ranging applications. [Display omitted] • Sustainable exploitation of Codium sp. within a bio-circular economy concept. • Extract bioactives with antioxidant, anti-inflammatory and photoprotective properties. • Seaweed by-products yield biochar by conventional and advanced processes. • Biochars from acid-mediated carbonization enhanced soil water retention capacity. [ABSTRACT FROM AUTHOR]