5 Alpha-Reductase Inhibitory Potential of Solanum nigrum Effective for Management of Alopecia.

The condition known as benign prostatic hyperplasia, which causes enlargement in the prostate gland and male pattern hair loss are both treated with a family of drugs known as 5 alpha reductase inhibitors. This study shows that Solanum nigrum L. has 5 alpha reductase inhibitory action, that is helpful in the treatment of androgenic diseases. It was carried out to screen for phytochemicals and to analyze high-performance thin layer chromatography. Using petroleum ether and methanol extract, the 5 alpha reductase inhibitory activity of the plant was compared to the renowned 5 alpha-reductase inhibitor finasteride. The inhibition of extracts to the enzyme was assessed using a biochemical approach to measure the activity of 5 alpha-reductase. Because the substrate nicotinamide adenine dinucleotide phosphate has a particular absorbance at 340 nm, the optical density value of each sample was monitored with an ultraviolet spectrophotometer. Nicotinamide adenine dinucleotide phosphate concentration rises over time in the presence of a 5 alpha-reductase inhibitor because the enzyme 5 alpha-reductase needs nicotinamide adenine dinucleotide phosphate as a substrate. Thus, this technique implies 5 alpha-reductase activity. The method was quite successful in evaluating the plant capacity to block 5 alpha-reductase. It was determined that Solanum nigrum L. petroleum ether extract 125.528±0.634 (µg/ml) and linoleic acid (chemical biomarker of the plant material) 89.672±0.669 (µg/ml) were promising candidates for future investigation into their anti-androgenic activities. [ABSTRACT FROM AUTHOR]