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Adhesion of Klebsiella oxytoca to bladder or lung epithelial cells is promoted by the presence of other opportunistic pathogens.

Authors :
Giliazeva, Adeliia
Akosah, Yaw
Noack, Jonas
Mardanova, Ayslu
Source :
Microbial Pathogenesis. May2024, Vol. 190, pN.PAG-N.PAG. 1p.
Publication Year :
2024

Abstract

The intestinal and respiratory tracts of healthy individuals serve as habitats for a diverse array of microorganisms, among which Klebsiella oxytoca holds significance as a causative agent in numerous community- and hospital-acquired infections, often manifesting in polymicrobial contexts. In specific circumstances, K. oxytoca , alongside other constituents of the gut microbiota, undergoes translocation to distinct physiological niches. In these new environments, it engages in close interactions with other microbial community members. As this interaction may progress to co-infection where the virulence of involved pathogens may be promoted and enhance disease severity, we investigated how K. oxytoca affects the adhesion of commonly co-isolated bacteria and vice versa during co-incubation of different biotic and abiotic surfaces. Co-incubation was beneficial for the adhesion of at least one of the two co-cultured strains. K. oxytoca enhanced the adhesion of other enterobacteria strains to polystyrene and adhered more efficiently to bladder or lung epithelial cell lines in the presence of most enterobacteria strains and S. aureus. This effect was accompanied by bacterial coaggregation mediated by carbohydrate-protein interactions occurring between bacteria. These interactions occur only in sessile, but not planktonic populations, and depend on the features of the surface. The data are of particular importance for the risk assessment of the urinary and respiratory tract infections caused by K. oxytoca , including those device-associated. In this paper, we present the first report on K. oxytoca ability to acquire increased adhesive capacities on epithelial cells through interactions with common causal agents of urinary and respiratory tract infections. [Display omitted] • An inaugural study on adhesion dynamics in mixed cultures with K. oxytoca and other opportunistic pathogens. • K. oxytoca promotes the adhesion of Escherichia coli , Enterobacter cloacae , and other Klebsiella spp. to polystyrene. • On epithelial cells, the adhesion of K. oxytoca is increased by staphylococci, E. coli , E. cloacae , and other Klebsiella spp. • In mixed cultures, K. oxytoca coaggregates with other bacteria during adhesion. • The impact of bacterial surface carbohydrates and proteins in adhesion and coaggregation is cell-type dependent. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
08824010
Volume :
190
Database :
Academic Search Index
Journal :
Microbial Pathogenesis
Publication Type :
Academic Journal
Accession number :
176760717
Full Text :
https://doi.org/10.1016/j.micpath.2024.106642