Farklı Dozlarda Uygulanan Streptozotosinin Sıçan Karaciğerine Etkilerinin Stereolojik Metotlarla İncelenmesi.

Diabetes mellitus is a group of chronic metabolic conditions, all of which are characterized by elevated blood glucose levels resulting from the body's inability to produce insulin or resistance to insulin action, or both. Insulin is the main hormone that regulates glucose production and use by body tissues and organs. Diabetes affects all systems in the body, including the liver. Streptozotocin is an antibiotic that performs pancreatic β-cell destruction and is widely used to induce experimental diabetes. In this study; It was aimed to calculate the volume and cell numbers of the morphometric changes caused by streptozotocin-induced diabetes in the rat liver using stereological methods. Twenty adult male rats were used in the study. Four groups were created randomly. Blood glucose values of all groups were measured. Diabetes was induced by administering different doses of streptozotocin (55 mg/kg and 65 mg/kg). After 48 hours, blood glucose values were measured again. Four weeks later, liver tissues of all rats from the control and experimental groups were taken under anesthesia and fixed in 10% formaldehyde. The fixed liver tissues were divided into equal parts by physical fractionation. It was fixed in paraffin after passing through dehydration stages. Histological sections were prepared. It was stained with Hematoxylin-Eosin, examined under a light microscope and photographed. The total volume of the liver was calculated using the Cavalieri Principle and the number of hepatocytes was calculated with the unbiased counting frame of the dissector method. In the diabetes-related groups, the increase in liver volume and cell number was significant compared to the control group (p<0.05). As a result, streptozotocin injection and the resulting diabetes lead to an increase in liver volume and hepatocyte numbers. [ABSTRACT FROM AUTHOR]