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Interleukin 4 induces the expression of inducible nitric oxide synthase in eosinophils

Interleukin 4 induces the expression of inducible nitric oxide synthase in eosinophils

Authors :
Paoliello-Paschoalato, A.B.
Oliveira, S.H.P.
Cunha, F.Q.
Source :
Cytokine. May2005, Vol. 30 Issue 3, p116-124. 9p.
Publication Year :
2005

Abstract

Abstract: We investigated the effects of the Th2-like cytokines IL-4 and IL-13 and of IL-10 on the induction of iNOS and NO production in rat eosinophils. Addition of mIL-4 to the eosinophil culture increased iNOS activity and nitrite production but did not improve the stimulatory effect of IFN-γ and LPS. In contrast to eosinophils, addition of mIL-4 to macrophage cultures inhibited the iNOS expression and nitrite production induced by IFN-γ plus LPS. Addition of mIL-13 to the eosinophil cultures did not significantly change iNOS activity and nitrite production in cells stimulated or not with IFN-γ plus LPS. On the other hand, IL-13 inhibited iNOS activity in IFN-γ plus LPS-stimulated macrophages. In the presence of IL-10, iNOS activity in non-stimulated eosinophil or macrophage cultures was not significantly altered, but the enzyme expression was inhibited in IFN-γ plus LPS-stimulated eosinophils or macrophages. The production of nitrite by eosinophils stimulated by IFN-γ plus LPS was inhibited by the presence of IL-10 in the medium. In conclusion, eosinophils might exhibit differential modulation of the l-arginine/iNOS pathway depending on the profile of Th2 cytokines produced during allergic diseases. IL-4 appears to be an important Th2 cytokine involved in the induction of the l-arginine/iNOS pathway in eosinophils. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
10434666
Volume :
30
Issue :
3
Database :
Academic Search Index
Journal :
Cytokine
Publication Type :
Academic Journal
Accession number :
17664993
Full Text :
https://doi.org/10.1016/j.cyto.2005.01.001