马铃薯 WOX 基因家族的鉴定及在离体再生和非生物胁 迫中的表达分析.

[Objective] The WUSCHEL-related homeobox(WOX)gene family is plant-specific, which plays important roles in plant growth and development, regulation of stem cell differentiation, and response to adverse stress. Identification of potato WOX gene family may provide a theoretical basis and gene resources for potato genetic improvement.[Method] Based on the WOX protein sequences of Arabidopsis thaliana, tomato, tobacco and rice, all members of potato WOX gene family were identified by HMMER 3.0 and BLASTP, MCScanX was used for collinear analysis, and the phylogenetic tree was constructed using neighbor-joining method. Physical and chemical properties, gene structure, protein motif, and transcription factor binding sites of potato WOX members were analyzed by ExPASy, GSDS and other software. Expression patterns of StWOXs were analyzed based on potato transcriptome data from PGSC database. RT-qPCR assay was carried out to analyze relative expression of StWOX5 in four potato varieties(lines)with different in vitro regeneration abilities.[Result] 11 members of potato WOX gene family were identified, distributed on five chromosomes, and classified into three clades, namely WUS, intermediate and ancient, with differences in gene structure, motif composition, transcription factor binding sites. The results showed that members of potato WOX gene family were involved in in vitro regeneration and abiotic stress responses. StWOX4/5/11/13 were specifically expressed in callus, StWUS and StWOX1/3c/4/5/13 were up-regulated under mannitol treatment, StWOX3a/11 were up-regulated under salt treatment, and StWOX4/5/13 were up-regulated under heat stress. The relative expression of StWOX5 was positively correlated with the differentiation rate.[Conclusion] Different potato WOX genes had potential functional diversity. StWOX5 may promote potato regeneration in vitro and participate in abiotic stress responses. [ABSTRACT FROM AUTHOR]