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Lidocaine inhibits migration of tenocytes by downregulating focal adhesion kinase and paxillin phosphorylation.

Authors :
Chang, Hsiang‐Ning
Chen, Chih‐Kuang
Yu, Tung‐Yang
Pang, Jong‐Hwei S.
Hsu, Chih‐Chin
Lin, Li‐Ping
Tsai, Wen‐Chung
Source :
Journal of Orthopaedic Research. May2024, Vol. 42 Issue 5, p985-992. 8p.
Publication Year :
2024

Abstract

Lidocaine is the most frequently applied local infiltration anesthetic agent for treating tendinopathies. However, studies have discovered lidocaine to negatively affect tendon healing. In the current study, the molecular mechanisms and effects of lidocaine on tenocyte migration were evaluated. We treated tenocytes intrinsic to the Achilles tendons of Sprague–Dawley rats with lidocaine. The migration ability of cells was analyzed using electric cell‐substrate impedance sensing (ECIS) and scratch wound assay. We then used a microscope to evaluate the cell spread. We assessed filamentous actin (F‐actin) cytoskeleton formation through immunofluorescence staining. In addition, we used Western blot analysis to analyze the expression of phospho‐focal adhesion kinase (FAK), FAK, phospho‐paxillin, paxillin, and F‐actin. We discovered that lidocaine had an inhibitory effect on the migration of tenocytes in the scratch wound assay and on the ECIS chip. Lidocaine treatment suppressed cell spreading and changed the cell morphology and F‐actin distribution. Lidocaine reduced F‐actin formation in the tenocyte during cell spreading; furthermore, it inhibited phospho‐FAK, F‐actin, and phospho‐paxillin expression in the tenocytes. Our study revealed that lidocaine inhibits the spread and migration of tenocytes. The molecular mechanism potentially underlying this effect is downregulation of F‐actin, phospho‐FAK, and phospho‐paxillin expression when cells are treated with lidocaine. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
07360266
Volume :
42
Issue :
5
Database :
Academic Search Index
Journal :
Journal of Orthopaedic Research
Publication Type :
Academic Journal
Accession number :
176535436
Full Text :
https://doi.org/10.1002/jor.25762