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Bergamot regulates oxidized low-density lipoprotein-induced inflammation and foam cell formation of human umbilical vein endothelial cells by regulating SIRT1/NF-κB pathway.

Authors :
Fan Zhao
Taimin Liu
Bo Liu
Jun Yin
Source :
Tropical Journal of Pharmaceutical Research. Mar2024, Vol. 23 Issue 3, p485-490. 6p.
Publication Year :
2024

Abstract

Purpose: To investigate the effects of bergamot (BGM) on the progression of atherosclerosis, and to unravel the mechanism of action. Methods: Oxidized low-density lipoprotein (Ox-LDL)-induced HUVECs were used as an in vitro model of atherosclerosis. CCK-8, flow cytometry (FCM), and enzyme-linked Immunosorbent assay (ELISA) assays were performed to confirm the effects of BGM on the viability and inflammation of ox-LDLinduced HUVECs. Oil-red staining and immunoblot tests were conducted to determine the effects of BGM on foam cell formation and macrophage polarization. Furthermore, The mechanism of action of BGM was examined by immunoblot studies. Results: BGM alleviated the ox-LDL-stimulated decline in HUVEC cell viability, and the ox-LDLstimulated HUVEC inflammation, but inhibited ox-LDL-stimulated foam cell formation and macrophage polarization in vitro (p < 0.05). In addition, BGM regulated SIRT1/NF-κB pathway, thereby alleviating atherosclerosis (p < 0.05). Conclusion: BGM regulates OX-LDL-induced inflammation and foam cell formation of HUVECs by mediating SIRT1/NF-κB pathway, and therefore can potentially serve as a drug for the treatment of atherosclerosis. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
15965996
Volume :
23
Issue :
3
Database :
Academic Search Index
Journal :
Tropical Journal of Pharmaceutical Research
Publication Type :
Academic Journal
Accession number :
176526175
Full Text :
https://doi.org/10.4314/tjpr.v23i3.1