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FaMYB6-like negatively regulates FaMYB10-induced anthocyanin accumulation during strawberry fruit ripening.
- Source :
-
Postharvest Biology & Technology . Jun2024, Vol. 212, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- Anthocyanins are one of the essential compounds affecting the quality of strawberry fruit. Several R2R3-MYB transcription factors have been well characterized as the anthocyanin activators in strawberry. However, we still lack valuable information on how R2R3-MYB repressors regulate strawberry anthocyanin accumulation. Here, we cloned a novel R2R3-MYB gene, FaMYB6-like , and characterized its repressive role during anthocyanin accumulation. FaMYB6-like encodes a nuclear localization protein, which was clustered into the FaMYB1-like type repressor clade in phylogenetic analysis. FaMYB6-like showed high expressions in anthocyanin-accumulating fruit and low transcript abundance in flowers, vegetative tissues, and early development stages of fruit. Transient overexpression of FaMYB6L-like repressed the expression of anthocyanin biosynthetic and transport genes, and reduced anthocyanin content in postharvest strawberry fruit. Transgenic tobacco lines overexpressing FaMYB6-like displayed a reduction in petal pigmentation. Overexpression of FaMYB6L-like in white-fleshed strawberry inhibited anthocyanin accumulation induced by FaMYB10. Protein interaction and promoter activation assays revealed that FaMYB6-like repressed the activation of target gene promoters by FaMYB10-FabHLH3 activation complex via its interaction with FabHLH3. Additionally, FaMYB6-like promoter activity can be activated by FaMYB10-FabHLH3 complex. Taken together, our study revealed that FaMYB6-like acted as a transcriptional repressor involved in an 'activator-and-repressor' feedback regulation mechanism for maintaining the balance of anthocyanin concentrations. • A new anthocyanin repressor, FaMYB6-like , was isolated from cultivated strawberry. • FaMYB6-like was highly expressed in anthocyanin-pigmented fruits. • Transgenic tobacco lines overexpressing FaMYB6-like displayed a reduction in petal pigmentation. • FaMYB6-like acts as the anthocyanin repressor by inhibiting MBW activity. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09255214
- Volume :
- 212
- Database :
- Academic Search Index
- Journal :
- Postharvest Biology & Technology
- Publication Type :
- Academic Journal
- Accession number :
- 176467052
- Full Text :
- https://doi.org/10.1016/j.postharvbio.2024.112891