莲草直胸跳甲 3 个气味结合蛋白基因的克隆与表达分析.

Odorant binding protein(OBP) is an important protein in the process of insect olfactory communication. OBPs are involved in the first step of insect odor recognition, and play an important role in insect activities such as foraging, aggregation, courtship, and looking for spawning places. In order to determine the expression characteristics of OBP genes in Agasicles hygrophila and to preliminarily understand its functions, in this study, three OBP genes were cloned and analyzed using bioinformatics. The relative expression levels of OBPs in different tissues were analyzed by quantitative real-time PCR. The results showed that the open reading frame of AhygOBP27, AhygOBP32, and AhygOBP44 were 351, 408, 423 bp, respectively. The proteins encoded by the three OBPs were acidic soluble proteins with small molecular weight. AhygOBP27 was mainly expressed in the antennae, head, feet and wings of both sexes. AhygOBP32 was expressed in antennae, head, leg and wings of the male and in antennae, feet and wings of the female. AhygOBP44 was expressed in the antennae, heads and feet of both sexes. The expression levels of above three genes in the antennae of male and female were significantly higher than that of other tissues. The expression level of AhygOBP27 and AhygOBP44 in female antennae were significantly higher than that in male antennae, and the expression level of AhygOBP32 in the antennae of males was significantly higher than that of females. The three genes might play an important role in the olfactory recognition of Agasicles hygrophila, AhypOBP27, and AhygOBP44 might be related to the recognition of host plant volatiles and the search for spawning ground; AhygOBP32 might be involved in the recognition of sex pheromone emitted by females. [ABSTRACT FROM AUTHOR]