生防菌株 JT86 对 3 种烟草产菌核病原菌的抑制效果研究.

[Objective] To explore the inhibitory effect of hiocontrol strain JT86 on sclerotia-producing pathogens of tobacco. [Methods] The cell wall hydrolase activities in the fementation broth of Pseudomonas aeruginosa 186 were detected using the double-antibody sandwich method. The mycelium and sclerotia morphological changes of the pathogen treated with JT86 fermentation broth were observed using scanning electron microscopy. The inhibitory effects on the hyphal growth and clematia germination of Rhizoctonia solani, Sclerotium ralf, and Sclerotinia sclerotiarum were tested. [Results] The inhibitory rates of JT86 fermentation broth on the hyphal growth of R. salani 5. rolfsii and S. sclerotiaru w 77.07% 50.82% and 95.25% respectively. After 24 h treatment with JT86 fermentation broth, the sclerotia of the three pathogens did not germinate, with inhibitory rates all reached 100% JT86 fermentation houth had high protease, cellulase and chitinase activities, but did not produce indoleacetic acid. Scanning electron microscopy showed that JT86 fermentation broth could cause severe hyphal atrophy and deformity of R. zolani. [Conclusion] The JT86 fermentation both had good inhibitory effects on the growth and development, as well as selestial germination of R. solani, S. ralfiii and S. scleratiorum. [ABSTRACT FROM AUTHOR]