马钱子苷通过肠道菌群和神经肽抑制 OVX 小鼠骨量 丢失的机制探讨.

Objective To observe the changes of bone mass, gut microbiota and neuropeptides in ovariectomized mice treated with Loganin. Methods 28 female C57BL/6J mice were randomly divided into sham operation group (SHAM), ovariectomized group (OVX), Loganin low-dose group (Loganin-L) and Loganin high-dose group (Loganin-H), with 7 mice in each group. Loganin-L and Loganin-H groups received Loganin 5mg / (kgd) and 20 mg/(kg.d) via oral gavage, respectively, while SHAM and OVX groups received the same dose of distilled water via oral gavage. After 12 weeks of intervention, BMD, BV/TV, Tb. N and Tb. Sp of the left femur were detected by Micro-CT. CTX, TRAP, PINP, TNF-α, IL-6, IL-10, CGRP, SP, VIP, NPY and LPS were detected by ELISA. 16 S rRNA high-throughput sequencing was used to detect the gut microbiota. Results BMD, BV/TV, Tb. N, P1NP, IL-10, CGRP, SP, VIP and NPY were significantly decreased in OVX group compared with SHAM group ( P < 0.05, P < 0, 1 ) ; Tb. Sp, CTX, TRAP, TNF-α, IL-6 and LPS were significantly increased in OVX group (P< 0.05, P < 0.01 . BMD, BV/TV, Tb. N, PINP, IL-10, CGRP, SP, VIP, and NPY of Loganin-L group tended to increase compared with OVX group (P > 0.05), while Tb. Sp CTX, TRAP, TNF-α, IL-6 and LPS tended to decrease (P > 0.05) . BMD, BV/TV, Tb. N, PINP, IL-10, CGRP, SP, VIP and NPY were significantly increased in Loganin-H group compared with OVX group (P<0.05); Tb. Sp, CTX, TRAP, TNF-α, IL-6 and LPS were significantly decreased (P < 0.05) At the phylum level, F/ B ratio was significantly decreased in OVX group ( P < 0.05 ) compared with SHAM group. However the F/B ratio in Loganin-L group has an increasing trend compared with OVX group (P > 0.05), and it was significantly increased in Loganin-H group (P< 0.05). At the genus level, norank f_Muribaculaceae exhibited a significant increase ( P < 0 ) while Lactobacillus demonstrated a significant decrease ( P < 0.05 ) in OVX group compared with SHAM group. Compared with OVX group, norank_f_Muribaculaceae tended to decrease in Loganin-L group ( P > 0.05, and Lactobacillustended to increase (P > 0.05) . Norank f_Muribaculaceae ( p < 0.01 showed a significant decrease (P < 0.05) whereas Lactobacillus exhibited a significant increase in Loganin-H group. Conclusion Loganin can inhibit bone loss in OVX mice in a dose-dependent manner, which may be achieved by reducing inflammatory response, increasing the level of neuropeptides and regulating the abundance of norank f_ Muribaculaceae and Lactobacillus. [ABSTRACT FROM AUTHOR]