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Plant-originated glycoprotein, G-120, inhibits the growth of MCF-7 cells and induces their apoptosis
- Source :
-
Food & Chemical Toxicology . Jun2005, Vol. 43 Issue 6, p961-968. 8p. - Publication Year :
- 2005
-
Abstract
- Abstract: We characterized the biological function of G-120, glycoprotein isolated from the ethanol extract of the herb, Ulmus davidiana Nakai (UDN). G-120 has anti-tumor activity and significantly inhibited proliferation of MCF-7 cells, as measured by the thymidine uptake assay. In addition, MTT and trypan blue exclusion experiments showed that the G-120-mediated inhibition of DNA synthesis may be due to a cytostatic, rather than a cytotoxic effect. Further studies of DNA analysis and propidium iodide staining revealed that G-120 induces apoptosis in MCF-7 cells. Interestingly, G-120 (100μg/ml) completely suppressed the binding of NF-κB to DNA and increased the cytosolic level of IκBα which prevented nuclear translocation of NF-κB. In addition, G-120 increased the expression of c-Jun, Fra-1, and Fra-2, but did not affect the expression of c-Fos. Collectively, it is believed that G-120 exerts an important role in the induction of apoptosis, suppression of NF-κB activation, and induction of c-Jun/Fra-1 or c-Jun/Fra-2 dimerization in MCF-7 cells. Consequently, G-120 could be considered as an anti-cancer agent, although further detailed experiments should be performed. [Copyright &y& Elsevier]
- Subjects :
- *GLYCOPROTEINS
*HERBS
*ALCOHOL
*CELLS
*APOPTOSIS
Subjects
Details
- Language :
- English
- ISSN :
- 02786915
- Volume :
- 43
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Food & Chemical Toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 17639613
- Full Text :
- https://doi.org/10.1016/j.fct.2005.02.002