Molecular analysis of the role of polymerase theta in gene targeting in Arabidopsis thaliana.

SUMMARY: Precise genetic modification can be achieved via a sequence homology‐mediated process known as gene targeting (GT). Whilst established for genome engineering purposes, the application of GT in plants still suffers from a low efficiency for which an explanation is currently lacking. Recently reported reduced rates of GT in A. thaliana deficient in polymerase theta (Polθ), a core component of theta‐mediated end joining (TMEJ) of DNA breaks, have led to the suggestion of a direct involvement of this enzyme in the homology‐directed process. Here, by monitoring homology‐driven gene conversion in plants with CRISPR reagent and donor sequences pre‐integrated at random sites in the genome (in planta GT), we demonstrate that Polθ action is not required for GT, but instead suppresses the process, likely by promoting the repair of the DNA break by end‐joining. This finding indicates that lack of donor integration explains the previously established reduced GT rates seen upon transformation of Polθ‐deficient plants. Our study additionally provides insight into ectopic gene targeting (EGT), recombination events between donor and target that do not map to the target locus. EGT, which occurs at similar frequencies as "true" GT during transformation, was rare in our in planta GT experiments arguing that EGT predominantly results from target locus recombination with nonintegrated T‐DNA molecules. By describing mechanistic features of GT our study provides directions for the improvement of precise genetic modification of plants. [ABSTRACT FROM AUTHOR]