Back to Search
Start Over
A Magnetic Nanoprobe Technology for Detecting Molecular Interactions in Live Cells.
- Source :
-
Science . Jul/Aug2005, Vol. 309 Issue 5731, p121-125. 5p. - Publication Year :
- 2005
-
Abstract
- Technologies to assess the molecular targets of biomolecules inliving cells are lacking. We have developed a technology calledmagnetism-based interaction capture (MAGIC) that identifies moleculartargets on the basis of induced movement of superparamagneticnanoparticles inside living cells. Efficient intracellular uptake ofsuperparamagnetic nanoparticles (coated with a small molecule ofinterest) was mediated by a transducible fusogenic peptide. Thesenanoprobes captured the small molecule's labeled target protein and weretranslocated in a direction specified by the magnetic field. Use ofMAGIC in genome-wide expression screening identified multiple proteintargets of a drug. MAGIC was also used to monitor signal-dependentmodification and multiple interactions of proteins. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BIOMOLECULES
*CELLS
*NANOPARTICLES
*PARAMAGNETISM
*PEPTIDES
*GENE expression
Subjects
Details
- Language :
- English
- ISSN :
- 00368075
- Volume :
- 309
- Issue :
- 5731
- Database :
- Academic Search Index
- Journal :
- Science
- Publication Type :
- Academic Journal
- Accession number :
- 17618937
- Full Text :
- https://doi.org/10.1126/science.1112869