2 型糖尿病足患者肠道菌群特征及与临床生化指标相关性研究.

16S rRNA sequencing technology was used to detect the intestinal flora of the research subjects, explore the intestinal flora characteristics and its correlation with Clinical biochemical index in patients wihe type 2 diabetic foot, and provide a new theoretical basis for the treatment of diabetic foot based on intestinal flora. The research objects were divided into three groups: diabetic foot group (A, n=32), diabetic group (B, n=32), and control group (C, n=31), and collected fecal samples and clinical biochemical indexes such as fasting blood glucose, HbA1c and inflammatory indexes. 16S rRNA sequencing technology was used to detect the intestinal flora of the objects and analyze the intestinal flora characteristics of patients with type 2 diabetic foot and its correlation with clinical biochemical indexes. 1. Compared with the diabetic group and control group, blood glucose, lipid levels and inflammatory indexes were significantly increased in the diabetic foot group (P<0.05). 2. The species richness of the diabetic foot group was significantly lower than that of the other two groups (P<0.05), and there were differences in alpha diversity and 茁 diversity among the three groups (P<0.05). 3. In the diabetic foot group, the abundance of harmful iconic species such as Vibrio (especially desulfovibrio), Coprococcus and anaerobic Claviculus increased compared with the other two groups, while the beneficial iconic species such as bifidobacterium, Akkermania, Coprobiotic and Lactobacillus decreased compared with the other two groups. 5.Spearman correlation analysis showed that Ruminococcus was negatively correlated with FPG, HbA1c, WBC, NE%, CRP and ESR (P<0.05), while Shigella was positively correlated with WBC, NE%, CRP and ESR (P<0.05). Desulfovibrio was positively correlated with FPG, HbA1c, TG, WBC, NE% and CRP (P<0.05). The intestinal flora in the diabetic foot group was more disordered, and the characteristics of the disordered intestinal flora were associated with more disordered glucose metabolism, lipid metabolism and more severe inflammation in the diabetic foot group. Therefore, intestinal flora disorders may be closely related to the occurrence and development of type 2 diabetic foot. [ABSTRACT FROM AUTHOR]