硫酸胆固醇对桥本氏甲状腺炎小鼠模型的治疗效果及相关机制研究.

Objective To investigate the therapeutic effect of cholesterol sulfate(CS)on the Hashimoto's thyroiditis mouse model. Methods Female NOD. H-2h4 mice were fed with 0.05% NaI in different periods and treated with CS by intraperitoneal injection for two consecutive weeks. HE staining was used to visualize and score the degree of lymphocyte infiltration in the thyroid; serum levels of thyroglobulin antibody(TgAb), thyroid peroxidase antibody(TPOAb), thyroxine(T4), and thyroid stimulating hormone(TSH)were detected by ELISA. The proportions of B cells and Treg, Th17, Th1, and Th2 cells were analyzed by immunofluorescence staining flow cytometry. Results HE staining showed that the inflammatory score of thyroid tissue in mice after intraperitoneal injection of CS in the 8-week group and the 16-week group decreased significantly(P<0.05). In the 64-week group, there was no significant difference between the treatment group and the induction group(P=0.31). Serological analysis showed that after CS intervention, the levels of TgAb and TPOAb in mice induced by 0.05% NaI significantly lowered(P<0.05)in the 8-week group and the 16-week group, while thyroid function(TSH and T4 levels)of the mice changed significantly only in the 16-week group. Flow cytometry analysis showed that in the 8-week group, after CS intervention the proportions of B lymphocytes and Th1, Th2, Th17 and Treg cells in mice were significantly changed(P<0.05). Conclusion CS has significant therapeutic and remission effects on the early and middle stages of Hashimoto's thyroiditis. [ABSTRACT FROM AUTHOR]