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Cross-regulation between proteome reallocation and metabolic flux redistribution governs bacterial growth transition kinetics.
- Source :
-
Metabolic Engineering . Mar2024, Vol. 82, p60-68. 9p. - Publication Year :
- 2024
-
Abstract
- Bacteria need to adjust their metabolism and protein synthesis simultaneously to adapt to changing nutrient conditions. It's still a grand challenge to predict how cells coordinate such adaptation due to the cross-regulation between the metabolic fluxes and the protein synthesis. Here we developed a dynamic Constrained Allocation Flux Balance Analysis method (dCAFBA), which integrates flux-controlled proteome allocation and protein limited flux balance analysis. This framework can predict the redistribution dynamics of metabolic fluxes without requiring detailed enzyme parameters. We reveal that during nutrient up-shifts, the calculated metabolic fluxes change in agreement with experimental measurements of enzyme protein dynamics. During nutrient down-shifts, we uncover a switch of metabolic bottleneck from carbon uptake proteins to metabolic enzymes, which disrupts the coordination between metabolic flux and their enzyme abundance. Our method provides a quantitative framework to investigate cellular metabolism under varying environments and reveals insights into bacterial adaptation strategies. • A dynamic model integrating protein allocation with flux balance analysis. • Model can predict transition kinetics of metabolic fluxes without enzymatic details. • Enzyme protein dynamics determine metabolic fluxes kinetics during nutrient upshift. • Switch of metabolic bottleneck during nutrient downshift. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10967176
- Volume :
- 82
- Database :
- Academic Search Index
- Journal :
- Metabolic Engineering
- Publication Type :
- Academic Journal
- Accession number :
- 176067243
- Full Text :
- https://doi.org/10.1016/j.ymben.2024.01.008