Antioxidant activity and chemical compound analysis of inoculated agarwood extract.

Gyrinops versteegii is an endemic plant in Nusa Tenggara Barat. The spesies have not been widely explored as a source of natural products. Biological activity screening of leaves, fruit barks, and stem barks from agarwood-producing plant have been investigated in 2016. The previous result revealed that beside of acetone extract from the leaves of the inoculated G. versteegii plant, the acetone extract from the trunk of agarwood showed high antioxidant activity. To complete the previous research, an isolation of compounds responsible for antioxidant activity from the extract of agarwood trunk is essential to study. Therefore, the purposes of this research are to obtain data and information of fraction having high bioactivity and to isolate antioxidant compounds from the trunk extract of agarwood. The isolation is conducted through extraction, fractionation and further analysis on antioxidant assay and GC-MS analysis. The research showed that etil acetate and methanol fractions had high antioxidant activity with IC50 104.69±6.07 ppm and 90.27±7.39 ppm respectively. But crude extract still has the lowest IC50. The lower antioxidant activity of the fractions of the crude extract can be considered because of the synergistic nature of the compounds contained in the crude extract, where there are more diverse compounds than the methanol fraction. Several compounds that may have potential as antioxidants from inoculated gaharu extract are 1-(benzyloxy)-8-Naphthol, 6-Methoxy-4-(methoxymethyl)-2-phenylquinoline, 5-hydroxy-4',7-dimethoxy-flavone, stigmastan-3,5-dien, (24Z)-ethylidenechilesterol, 3-Nitro-1,2-benzenedicarboxylic acid, 3-β-hydroxy-androst-5,16-ene. And 5-hydroxy-4',7-dimethoxy-flavone or apigenin 7,4'dimethyl ether is a methanol and ethyl acetate fraction compound with potential antioxidant activity. [ABSTRACT FROM AUTHOR]