Back to Search
Start Over
MicroRNA Sensors Based on CRISPR/Cas12a Technologies: Evolution From Indirect to Direct Detection.
- Source :
-
Critical Reviews in Analytical Chemistry . Mar2024, p1-17. 17p. 5 Illustrations, 2 Charts. - Publication Year :
- 2024
-
Abstract
- AbstractMicroRNA (miRNA) has emerged as a promising biomarker for disease diagnosis and a potential therapeutic targets for drug development. The detection of miRNA can serve as a noninvasive tool in diseases diagnosis and predicting diseases prognosis. CRISPR/Cas12a system has great potential in nucleic acid detection due to its high sensitivity and specificity, which has been developed to be a versatile tool for nucleic acid-based detection of targets in various fields. However, conversion from RNA to DNA with or without amplification operation is necessary for miRNA detection based on CRISPR/Cas12a system, because dsDNA containing PAM sequence or ssDNA is traditionally considered as the activator of Cas12a. Until recently, direct detection of miRNA by CRISPR/Cas12a system has been reported. In this review, we provide an overview of the evolution of biosensors based on CRISPR/Cas12a for miRNA detection from indirect to direct, which would be beneficial to the development of CRISPR/Cas12a-based sensors with better performance for direct detection of miRNA. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10408347
- Database :
- Academic Search Index
- Journal :
- Critical Reviews in Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 176030785
- Full Text :
- https://doi.org/10.1080/10408347.2024.2329229