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MiR-29a-3p mediates phosphatase and tensin homolog and inhibits osteoarthritis progression.
- Source :
-
Functional & Integrative Genomics . Apr2024, Vol. 24 Issue 2, p1-12. 12p. - Publication Year :
- 2024
-
Abstract
- Despite substantial progress in clinical trials of osteoarthritis (OA) gene therapy, the prevalence of OA is still on the rise. MiRNAs have a potential biomarker and therapeutic target for OA. OA cartilage and chondrosarcoma cells were studied to determine the role of miR-29a-3p and PTEN. OA cartilage and human chondrosarcoma cells (SW1353) were obtained. miR-29a-3p and PTEN signature expression was determined by RT-qPCR. The binding relationship between miR-29a-3p and PTEN was investigated by dual-luciferase reporter gene and western blot assay. TUNEL, immunohistochemistry, CCK-8, and flow cytometry were utilized to determine the proliferation and apoptosis of SW1353 cells. This study indicated downregulation of miR-29a-3p expression and upregulation of PTEN expression in human OA primary chondrocytes or OA tissue samples, compared with the normal cartilage cells or tissues. PTEN expression was negatively correlated with miR-29a-3p expression, and miR-29a-3p targeted PTEN mechanistically. miR-29a-3p reduced SW1353 cell activity and proliferation and promoted cell apoptosis. However, the aforementioned effects could be reversed by downregulating PTEN. miR-29a-3p can stimulate chondrocyte proliferation and inhibit apoptosis by inhibiting PTEN expression. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1438793X
- Volume :
- 24
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Functional & Integrative Genomics
- Publication Type :
- Academic Journal
- Accession number :
- 175997811
- Full Text :
- https://doi.org/10.1007/s10142-024-01327-w