Dynamic changes and quantification of defence enzymes activities on chemo-fortified chickpea (Cicer aritenum L.) against Fusarium oxysporum f. sp. ciceris.

In pot culture in glass house, we studied the chickpea disease management using synthetic elicitors and defence enzymes against Fusarium oxysporum f. sp. ciceris [MF803741] on chickpea cv. JAKI 9218. Six synthetic chemical elicitors (benzothiadiazole, salicylic acid, potassium silicate, potassium phosphate, humic acid and carbendazim) were used at 3-concentrations. (1.0, 2.0 and 3.0 mM) on test chemo-fortify chickpea cv. JAKI 9218. The changes in dynamics of enzyme activity were recorded from upto 24 days from sowing. Among these, salicylic acid fortified at a concentration of 3.0 mM enriched seeds had the lowest incidence of wilt at 17.3 %, 66.0 % reduction in disease and five defence proteins, namely 14, 29, 53, 67, and 83.4 kDa, and four enzyme isoforms. That is, PO1 to PO4 (peroxidase) were expressed; PPO1 to PPO4 (polyphenol oxidases) and three SOD1 to SOD3 (superoxide dismutase). Besides dynamic changes in enzyme activity, peroxidase (absorbance 1.50), polyphenol oxidase (absorbance 1.42), superoxide dismutase (7.60 units), and phenol content (19.24) μg catechol/min/gram tissue increased by 18 days. Hence, using the least quantity of elicitors enhanced and triggered the defence profiles in form of antioxidants, enzymes and proteins in chickpea against to wilt pathogen. [ABSTRACT FROM AUTHOR]