Enhancement of medium-chain fatty acids production from sludge anaerobic fermentation liquid under moderate sulfate reduction.

In recent years, there has been a marked increase in the production of excess sludge. Chain-elongation (CE) fermentation presents a promising approach for carbon resource recovery from sludge, enabling the transformation of carbon into medium-chain fatty acids (MCFAs). However, the impact of sulfate, commonly presents in sludge, on the CE process remains largely unexplored. In this study, batch tests for CE process of sludge anaerobic fermentation liquid (SAFL) under different SCOD/SO 4 2− ratios were performed. The moderate sulfate reduction under the optimum SCOD/SO 4 2− of 20:1 enhanced the n-caproate production, giving the maximum n-caproate concentration, selectivity and production rate of 5.49 g COD/L, 21.4% and 4.87 g COD/L/d, respectively. The excessive sulfate reduction under SCOD/SO 4 2− ≤ 5 completely inhibited the CE process, resulting in almost no n-caproate generation. The variations in n-caproate production under different conditions of SCOD/SO 4 2− were all well fitted with the modified Gompertz kinetic model. Alcaligenes and Ruminococcaceae_UCG-014 were the dominant genus-level biomarkers under moderate sulfate reduction (SCOD/SO 4 2− = 20), which enhanced the n-caproate production by increasing the generation of acetyl-CoA and the hydrolysis of difficult biodegradable substances in SAFL. The findings presented in this work elucidate a strategy and provide a theoretical framework for the further enhancement of MCFAs production from excess sludge. [Display omitted] • Moderate sulfate reduction enhanced n-caproate production from SAFL in CE. • Excessive sulfate reduction resulted in almost no n-caproate generation. • The kinetics of n-caproate production well fitted the modified Gompertz model. • Moderate sulfate reduction optimized the microbial community composition. • Multiple metabolic pathways were enhanced under moderate sulfate reduction. [ABSTRACT FROM AUTHOR]