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Generation of Stable Cell Lines of APP695 (K595N, M596L) Gene Over-Expression via Lentiviral Over-Expression System.
- Source :
-
Biology Bulletin . Jan2024, Vol. 51 Issue 1, p30-36. 7p. - Publication Year :
- 2024
-
Abstract
- There are now various theories on the pathophysiology of Alzheimer's disease (AD), the β-Amyloid (Aβ) cascade hypothesis has garnered a lot of interest. This text applied lentivirus-encapsulated plasmid to transfect APP695 (K595N, M596L) gene into SH-SY5Y cells and thereby obtain a stable cell line over-expressing Aβ. We used qPCR to monitor the mRNA expression of β-Amyloid Precursor Protein (APP) gene, β-Site APP Cleaving Enzyme 1 (BACE1) gene, we also used Western Blot to detect the APP protein, BACE1 protein, and Aβ1-42, respectively, in addition, the expression of Aβ1-42 in SH-SY5Y cell lysate and cell culture supernatant was measured by Enzyme-Linked Immunosorbent Assay (ELISA). The findings demonstrated APP gene over-expression following APP695 (K595N, M596L) gene transfection, and also indicated the successful establishment of the Swedish mutant cell model. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10623590
- Volume :
- 51
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Biology Bulletin
- Publication Type :
- Academic Journal
- Accession number :
- 175753489
- Full Text :
- https://doi.org/10.1134/S1062359023602586