酶固定化技术的最新研究进展.

As an efficient and safe biocatalyst, enzymes have been widely used in many fields such as food, medicine and environmental governance, but it is difficult to realize further industrial application due to the poor environmental stability of free enzymes. Enzyme immobilization technology helps to improve the tolerance of free enzymes to sensitive environments and the stability during operation, and greatly reduces the application cost. This paper reviews the development and current situation of immobilization technology in the past five years, and summarizes the research progress of different immobilization methods (including traditional immobilization methods such as adsorption method and binding method and new immobilization methods such as coimmobilization enzyme method) and immobilization carriers (including natural material carriers, composite carriers and nanocarriers) in various fields. In general, compared with free enzymes, the immobilized enzyme system has been significantly improved in terms of stability and reusability. However, there are some shortcomings, such as lower recovery rate after immobilization, cumbersome and costly carrier synthesis pathway, and imperfect mechanism of immobilization enzyme.Finally, the development direction of the technology in the future was put forward based on these shortcomings. [ABSTRACT FROM AUTHOR]