PRIMERJAVA METOD ZA IZOLACIJO RNA IZ KORENIN IN LISTOV HMELJA ZA ANALIZE IZRAŽANJA GENOV Z METODO RT-qPCR.

Hops (Humulus lupulus L.) is a dioecious perennial climbing plant that plays an important role in the brewing industry. Since it is a host plant for many pathogens, research at the RNA level is critical to understand the plant's response to pathogens and mechanisms of disease resistance. Obtaining sufficient amounts of high-quality RNA for molecular analysis is often difficult in plants because they contain metabolites such as phenols and polysaccharides. For this purpose, we compared five different methods for RNA isolation from hop leaves and roots, including two commercially available kits for RNA isolation - Monarch Total RNA Miniprep Kit (NEB) and SpectrumTM Plant Total RNA Isolation Kit (Sigma-Aldrich); and three noncommercial protocols. For all samples, the concentration and purity of the isolated RNA was determined using a spectrophotometer. With the non-commercial 'Hot borate' and CTAB methods, we achieved the highest concentrations and purity of isolated RNA in both leaves and roots of hops. When the samples were analysed using the RT-qPCR method, we found that they were suitable for further molecular analysis. [ABSTRACT FROM AUTHOR]