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Ultrasensitive detection of NSE employing a novel electrochemical immunosensor based on a conjugated copolymer.

Authors :
Aydın, Muhammet
Aydın, Elif Burcu
Sezgintürk, Mustafa Kemal
Source :
Analyst. 3/7/2024, Vol. 149 Issue 5, p1632-1644. 13p.
Publication Year :
2024

Abstract

In the current study a simple and highly specific label-free impedimetric neuron specific enolase (NSE) immunosensor based on a copolymer matrix-coated disposable electrode was designed and tested. The copolymer matrix was prepared using a very conductive EDOT monomer and semi-conductive thiophene-bearing epoxy groups (ThEp), and the combination of the two monomers enhanced the conductivity and protein loading capacity of the electrode surface. The P(ThEp-co-EDOT) copolymer matrix was prepared via a drop-casting process and anti-NSE recognition biomolecules were immobilized directly on the epoxy groups of the copolymer. After the coupling of NSE molecules on the P(ThEp-co-EDOT) copolymer matrix-coated electrode surface, the charge transfer resistance (Rct) of the biosensor changed dramatically. These changes in Rct were proportional to the NSE molecule amounts captured by anti-NSE molecules. Under optimized experimental conditions, the increment in the Rct value was proportional to the NSE concentration over a range of 0.01 to 25 pg mL−1 with a detection limit (LOD) of 2.98 × 10−3 pg mL−1. This copolymer-coated electrode provided a lower LOD than the other biosensors. In addition, the suggested electrochemical immuno-platform showed good selectivity, superior reproducibility, long-term stability, and high recovery of NSE in real serum (95.64–102.20%) and saliva (95.28–105.35%) samples. These results showed that the present system had great potential for electrochemical biosensing applications. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00032654
Volume :
149
Issue :
5
Database :
Academic Search Index
Journal :
Analyst
Publication Type :
Academic Journal
Accession number :
175641484
Full Text :
https://doi.org/10.1039/d3an01602a