Preparation and mechanical properties of biomimetic mineralization collagen for dopamine-polyacrylamide-induced small intestinal submucosa.

• A lab-built ion diffusion test on film was designed and developed successfully. • The morphology of hydroxyapatite crystal growth was similar to nature bone. • Lamellar hydroxyapatite was synthesized by dopamine-polyacrylamide-induced SIS. • SIS-based mineralized collagen was prepared to mimic bone basic unit microstructure. Bone-like lamellar hydroxyapatite(HAP) was successfully synthesized by biomimetic mineralization on small intestinal submucosa(SIS) induced by dopamine(DA)-polyacrylamide (PAM).HAP was uniformly grown by the method of "collagen fiber active site-regulated ion nucleation growth" on SIS film by a lab-built ion diffusion experimental system. The hydrophilic angles before and after SIS modification were 85.3 ± 1.3° and 35.7 ± 1.2°, respectively. The morphology of grow HAP crystals was similar to that of nature bone. Then, DA-PAM@SIS/HAP material was prepared to mimic bone components and basic unit microstructure of natural bones. The tensile strength of composites was about 12 MPa, which was 2.4 times higher than that of the unmineralized films. The mineralized film should be expected to be applied in the field of bone remodeling and bone tissue engineering. [ABSTRACT FROM AUTHOR]