二次脱钙法在临床病理骨组织 HE制片中的应用研究.

Objective To explore the application value of secondary decalcification in the hematoxylin-eosin（HE） pathological section of the osseous tissue in clinical pathology. Methods A total of 100 pieces of wax blocks with insufficient decalcification, bone tissue or calcified tissue were collected in this hospital from June 2022 to December 2022. The wax blocks were made into pathological section and divided into the control group and the experimental group according to the different decalcification methods. The control group used the conventional decalcification method, and the experimental group used secondary delcalcifiaction method. Finally, the quality of the two groups of pathological section was compared. Results The structural integrity of the HE pathological section, HE staining satisfaction, and excellent rate of pathological section in the experimental group were all higher than those of the control group. The differences were statistically significant（P＜0. 05）. Conclusion The second ary decalcification method has a high application value in HE pathological section of clinical pathological bone tissue. [ABSTRACT FROM AUTHOR]