Back to Search
Start Over
In situ bio-functionalization and cell adhesion in microfluidic devices
- Source :
-
Microelectronic Engineering . Mar2005, Vol. 78-79, p556-562. 7p. - Publication Year :
- 2005
-
Abstract
- Abstract: Protein immobilization is of great importance for the development of biosensors, immunoassays and microfluidic devices. In this work, we study the in situ bio-functionalization and cell adhesion in microfluidic channels. A way of covalently linking antibodies on the channel surface while keeping their bio-functionality and its application for efficient cell adhesion were shown. Soft lithography has been used to fabricate simple microfluidic devices and all chemical and biologic materials were injected into microfluidic channels for the in situ bio-functionalization and cell adhesion. Our results show that the established bio-processing protocol significantly enhances the protein immobilization on both glass and poly-dimethylsiloxane surfaces, thereby allowing one to develop a large number of applications. [Copyright &y& Elsevier]
- Subjects :
- *MICROFLUIDICS
*CELL adhesion
*BIOSENSORS
*IMMUNOASSAY
Subjects
Details
- Language :
- English
- ISSN :
- 01679317
- Volume :
- 78-79
- Database :
- Academic Search Index
- Journal :
- Microelectronic Engineering
- Publication Type :
- Academic Journal
- Accession number :
- 17553173
- Full Text :
- https://doi.org/10.1016/j.mee.2004.12.071