姜酚和百里香酚对白羽肉鸡小肠形态结构 和屏障功能的影响.

[Objectives]The study investigated the effects of dietary supplementation with gingerol, thymol and their combination on small intestinal morphology, immune function and intestinal barrier of white feather broilers. [Methods]A total of 200 1-day-old broilers were randomly divided into four groups with 5 replicates per group and 10 chickens per replicate as followings: the control group(Con group, basal diet), the gingerol group(Gin group, basal diet+60 mg·kg-1 gingerol), the thymol group(Thy group, basal diet+30 mg·kg-1 thymol)and the gingerol+thymol group(Gin+Thy group, basal diet+30 mg·kg-1 gingerol+15 mg·kg-1 thymol). On day 42 of the experimental period, the jejunum and ileum of broilers were collected to make sections and observed, and the mucosa of the jejunum and ileum were taken to determine the immunoglobulin content, cytokine content, antioxidant enzyme activities, tight junctions and antioxidant-related gene expression. [Results]The ileal villus height(VH)trended to increase(P=0.063)and the ratio of villus height/crypt depth(V/C)significantly increased(P<0.05)in Gin+Thy group compared with those in Con group. The secretory immunoglobulin A(sIgA)content in jejunal and ileal mucosa significantly increased(P<0.05), while the interleukin-6(IL-6)and interferon-γ(IFN-γ)content in jejunal mucosa, and the IL-6 and interleukin-1β(IL-1β)content in ileal mucosa significantly decreased(P<0.05)in Gin+Thy group compared with those in Con group. Total antioxidant capacity(T-AOC)and glutathione peroxidase(GSH-Px)activities in jejunal and ileal mucosa in Gin+Thy group significantly increased compared with Con group(P<0.05). The Gin+Thy group had a higher mRNA relative expression of nuclear factor E2-related factor 2(Nrf2), heme oxygenase 1(HO-1), glutathione peroxidase(GPX4)in jejunal mucosa, and a higher mRNA relative expression of Nrf2, superoxide dismutase 1(SOD1)and GPX4 in ileal mucosa than those in Con group(P<0.05). The mRNA relative expression level of Occludin in jejunal and ileal mucosa significantly increased in Gin+Thy group compared with Con group(P<0.05). [Conclusions]Dietary supplementation with the gingerol and thymol combination improved the antioxidant capacity and immune function, and enhanced intestinal health by improving intestinal morphology and intestinal barrier of broilers. [ABSTRACT FROM AUTHOR]