大约克公猪精液质量与粪便菌群的相关性分析.

[Objectives]The objective of this experiment was to study the microbial composition, testosterone and endotoxin content of feces, from boars with different semen quality, and analyze the correlation between semen quality and fecal microbiota, testosterone and endotoxin. It will provide a basis for improving semen quality by regulating gut microbiota in pig production. [Methods]According to semen quality parameters of all boars in the three months before the experiment, 33 York boars of similar age(479.33±2.85)and body condition were selected and divided into low semen quality group(LSQ, n=12)and high semen quality group(HSQ, n=21). Fresh feces of experimental boars were collected to detect the content of testosterone and endotoxin, and the microbial composition of feces was analyzed by 16S ribosomal RNA gene sequencing technique. [Results]The fecal testosterone level of boars in the HSQ group was significantly higher than that in the LSQ group(P<0.01), and there was no significant difference in endotoxin levels. There were 3 differential bacteria on family level and 5 differential bacteria on genera level in the boar fecal microbiota of the HSQ group and LSQ group. At the family level, Eubacterium_coprostanoligenes_group was significantly enriched in the LSQ group(P<0.05), Atopobiaceae and Akkermansiaceae were significantly enriched in the HSQ group(P<0.05); at the genus level, g_norank_f_Eubacterium_coprostanoligenes_group and Faecalibaculum were significantly enriched in the LSQ group(P<0.05)and g_norank_f_Atopobiaceae, Akkermansia and Clostridium_sensu_stricto_6 were significantly enriched in the HSQ group(P<0.05). There was a significant negative correlation between Eubacterium_coprostanoligenes_group and sperm motility and sperm density(P<0.01), and there was a negative correlation between Eubacterium_coprostanoligenes_group and fecal testosterone levels(P=0.11); there was a significant positive correlation between Atopobiaceae and sperm motility, sperm density and fecal testosterone levels(P<0.05); Akkermansiaceae significantly positively correlated with sperm motility(P<0.01)and sperm density(P<0.05), and positively correlated with fecal testosterone levels(P=0.09). [Conclusions]The results showed that there were differences in the fecal testosterone level and microbial composition of boars with different semen quality, and there were different degrees of correlations between boar fecal microbial structure and semen quality, fecal testosterone levels. The results provide a new theoretical support for improving boar semen quality by regulating gut microbiota. [ABSTRACT FROM AUTHOR]