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Efficient production of 1,2,4-butanetriol from corn cob hydrolysate by metabolically engineered Escherichia coli.
- Source :
-
Microbial Cell Factories . 2/12/2024, Vol. 23 Issue 1, p1-11. 11p. - Publication Year :
- 2024
-
Abstract
- Corn cob is a major waste mass-produced in corn agriculture. Corn cob hydrolysate containing xylose, arabinose, and glucose is the hydrolysis product of corn cob. Herein, a recombinant Escherichia coli strain BT-10 was constructed to transform corn cob hydrolysate into 1,2,4-butanetriol, a platform substance with diversified applications. To eliminate catabolite repression and enhance NADPH supply for alcohol dehydrogenase YqhD catalyzed 1,2,4-butanetriol generation, ptsG encoding glucose transporter EIICBGlc and pgi encoding phosphoglucose isomerase were deleted. With four heterologous enzymes including xylose dehydrogenase, xylonolactonase, xylonate dehydratase, α-ketoacid decarboxylase and endogenous YqhD, E. coli BT-10 can produce 36.63 g/L 1,2,4-butanetriol with a productivity of 1.14 g/[L·h] using xylose as substrate. When corn cob hydrolysate was used as the substrate, 43.4 g/L 1,2,4-butanetriol was generated with a productivity of 1.09 g/[L·h] and a yield of 0.9 mol/mol. With its desirable characteristics, E. coli BT-10 is a promising strain for commercial 1,2,4-butanetriol production. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14752859
- Volume :
- 23
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Microbial Cell Factories
- Publication Type :
- Academic Journal
- Accession number :
- 175389926
- Full Text :
- https://doi.org/10.1186/s12934-024-02317-0