Meiotic nuclear divisions 1 suppresses the proliferation and invasion of pancreatic cancer cells via regulating H2A.X variant histone.

Introduction: Among all malignant tumors of the digestive system, pancreatic carcinoma exhibits the highest mortality rate. Currently, prevention and effective treatment are urgent issues that need to be addressed. Methods: The study focused on meiotic nuclear divisions 1 (MND1), integrating data from the Gene Expression Profiling Interactive Analysis (GEPIA) database with prognostic survival analysis. Simultaneously, experiments at cellular level were employed to demonstrate the effect of MND1 on the proliferation and migration of PC. The small-molecule inhibitor of MND1 was used to suppress the migration of PC cells by knocking down MND1 using small interfering RNA (siRNA) in Patu-8988 and Panc1 cell lines. Results: The results of Cell Counting Kit-8 indicated that the suppression of MND1 resulted in a decrease in cell proliferation. Wound healing and Transwell assays revealed that MND1 knockdown reduced cell migration and invasion. Flow cytometry revealed that inhibiting MND1 hindered the cell cycle. Furthermore, MND1 could stimulate the proliferation, migration, and invasion of Patu-8988 and Panc1 cells by increasing the expression of MND1. Notably, MND1 had a positive effect on H2AFX expression in PC cells. Elevated MND1 expression suggests the low overall survival rate of individuals diagnosed with PC. Conclusion: These findings suggest that MND1 has the potential to be a gene with the ability to accurately diagnose and treat PC. [ABSTRACT FROM AUTHOR]