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Assessing the Agreement of Light Microscopic Evaluation of Oral Lichen Planus Lesions With Associated Direct Immunofluorescence Evaluation.
- Source :
-
Clinical Pathology . Jan-Dec2023, Vol. 16, p1-6. 6p. - Publication Year :
- 2023
-
Abstract
- Aim/objective: Assess agreement between light microscopy and direct immunofluorescence (DIF) for histopathologic evaluation of oral lichen planus (OLP). METHODS: Records evaluated included 60 OLP, 16 lichenoid mucositis (LM), and 56 non-OLP/non-LM cases. Cases had both light microscopic and DIF evaluations. Histopathologic parameters of OLP included: (1) hydropic degeneration of the basal cell layer, (2) band-like lymphocytic infiltrate immediately subjacent to the epithelium, and (3) presence of Civatte bodies. Two calibrated examiners independently assessed light microscopic features. Examiners reviewed cases with discordant diagnoses to determine a consensus diagnosis. Intra-rater reliability (IRR), sensitivity, specificity, positive, and negative predictive values (PPV and NPV) were determined. RESULTS: Of 132 patients, 72.7% were female, average age 61.9 (SD = 13.8). Most common sites were gingiva (37.9%), buccal mucosa (37.1%), and tongue (7.6%). IRR was 0.74 (95% CI: 0.40, 1.00) for the consensus diagnosis and 0.73 (95% CI: 0.39, 1.00) and 0.34 (95% CI: -0.03, 0.72) for the 2 examiners. Comparing consensus and definitive diagnoses: sensitivity of light microscopy: 0.32 (95% CI: 0.20, 0.45); specificity: 0.88 (95% CI: 0.78, 0.94); PPV: 0.68 (95% CI: 0.48, 0.84), and NPV: 0.61 (95% CI: 0.51, 0.70). Conclusion: Light microscopy alone is not a viable alternative to adjunctive DIF for diagnosis of OLP lesions. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CONSENSUS (Social sciences)
*STATISTICS
*MUCOSITIS
*PREDICTIVE tests
*CONFIDENCE intervals
*MICROSCOPY
*ORAL lichen planus
*ACQUISITION of data
*TONGUE
*FISHER exact test
*INTER-observer reliability
*LYMPHOCYTES
*EPITHELIUM
*COMPARATIVE studies
*T-test (Statistics)
*FLUORESCENT antibody technique
*MEDICAL records
*DESCRIPTIVE statistics
*CHI-squared test
*RESEARCH funding
*SENSITIVITY & specificity (Statistics)
*ORAL mucosa
*DATA analysis software
*GINGIVA
*EVALUATION
RESEARCH evaluation
Subjects
Details
- Language :
- English
- ISSN :
- 2632010X
- Volume :
- 16
- Database :
- Academic Search Index
- Journal :
- Clinical Pathology
- Publication Type :
- Academic Journal
- Accession number :
- 175290411
- Full Text :
- https://doi.org/10.1177/2632010X231197111