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Per-cell histone acetylation is associated with terminal differentiation in human T cells.

Authors :
Yang, Cheng
Li, You
Hu, Yaqiu
Li, Qian
Lan, Yinghua
Li, Yongguo
Source :
Clinical Epigenetics. 2/6/2024, Vol. 16 Issue 1, p1-10. 10p.
Publication Year :
2024

Abstract

Background: Epigenetic remodeling at effector gene loci has been reported to be critical in regulating T cell differentiation and function. However, efforts to investigate underlying epigenetic mechanisms that control T cell behaviors have been largely hindered by very limited experimental tools, especially in humans. Results: In this study, we employed a flow cytometric assay to analyze histone acetylation at single-cell level in human T cells. The data showed that histone acetylation was increased during T cell activation. Among T cell subsets, terminally differentiated effector memory T (TEMRA) cells robustly producing effector cytokines were hyper-acetylated. Conversely, these TEMRA cells had lower expression levels of TCF-1, a key transcription factor for maintaining stem cell features. Pharmaceutical inhibition of histone acetylation using a small molecule C646 restrained the production of effector molecules, but retained stem cell-like properties in T cells after expansion. Conclusions: Per-cell histone acetylation is associated with terminal differentiation and poor stemness in human T cells. These observations suggest a new approach to enhance the stem cell-like properties of T cells and improve the efficacy of immunotherapy. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
18687075
Volume :
16
Issue :
1
Database :
Academic Search Index
Journal :
Clinical Epigenetics
Publication Type :
Academic Journal
Accession number :
175278898
Full Text :
https://doi.org/10.1186/s13148-024-01634-w