The development of probiotics for reducing methane gas emission in Indonesian local beef cattle: In vitro assays.

In this study, the effect of some candidate's probiotics in reducing methane gas emission from feed digestion in vitro. Gas production was analyzed using a fermenter bottle with rumen fluid as an inoculant according to the Theodoru and Brooks' method. Rumen fluid from collected from two fistulated Peranakan Ongole (PO) Steers in Cibinong Science Center, Bogor. Rumen fluid was mixed with ANKOM fermentation buffer with a ratio of 1:4 in a 50 mL solution. Basal feed was 0.5g mixed ration consisting of 30% concentrate and 70% elephant grass based on dry matter (DM) content. Gas production, methane production, pH, and In Vitro True Digestibility (IVTD) were evaluated on 24 h and 48 h incubation periods. In the first experiment, Bacillus subtilis (P1), Lactobacillus plantarum (P2), new species NB4.11 (P3), Enterococcus faecalis A3-1 (P4), Enterococcus faecalis 13PS3 (P5) and combination of previous probiotics (P6) with concentration (each species) 109 CFU/mL were assayed for their mitigation effect. All the bacteria were isolated from Bali Cattle fed with oil palm by-products. The result showed there was no significant effect of probiotic addition to mitigate methane emission and feed digestibility. In experiment two, L. plantarum was chosen from experiment one and enriched with Acetobacterium woodii. The combination of L. plantarum and A. woodii showed significant prohibition action to methanogenesis as much as 18.76% compared with control treatment at 24 h incubation. The combination of L. plantarum and A. woodii are recommended as a potential probiotic to reduce greenhouse gasses in Indonesian beef cattle. [ABSTRACT FROM AUTHOR]