Coagulase and clumping factor in Staphylococcus aureus from Ettawa-Crossbreed goat (PE) mastitis in Yogyakarta Indonesia.

Mastitis is an inflammatory disease of the udder and it's divided into two types such as clinical mastitis (CM) and subclinical mastitis (SCM). Staphylococcus aureus is responsible for mastitis which produces coagulase (Coa) and clumping factor (Clf). The purpose of this study was to determine the ability to produce Coa and Clf in S. aureus isolated from CM and SCM in Ettawa-Crossbreed Goat (PE) in Yogyakarta. A total of 384 PE goats' lactation were used in this study. The case of CM was diagnosed based on the manifestation of visible signs such as inflammation of the udder characterized by heat and swelling with pain upon palpation, whereas SCM is based on the California mastitis test (CMT) score. CMT scores 0 and +1 were considered−while score +2 or +3 taken +. Isolation of S. aureus was carried out by using biochemical tests, whereas Coa by tube coagulation than Clf with slide agglutination test. A total of 384 PE goats collected, 29.17% showed SCM and 17.8% caused by S. aureus. On the other hand, 3.39% of PE goats showed CM, and 53.84% were caused by S. aureus. S. aureus isolates from CM have found coagulase-positive staphylococci (CoPS) 85.7%, coagulase-negative staphylococci (CoNS) 14.3%, Clf+71.43%, Clf−28.57%; whereas SCM has found CoPS 20%, CoNS 80%, Clf+15% and Clf−85%. The study showed that S. aureus with CoPS and Clf+were more common in CM than SCM in PE goats in Yogyakarta, Indonesia. [ABSTRACT FROM AUTHOR]