石珊瑚共附生真菌次级代谢产物的抗炎活性及化学多样 性研究.

Stony coral symbiotic fungi were used to investigate the effects of different culture conditions on the anti‑inflammatory activities and chemical diversities of their secondary metabolites. Based on the OSMAC (one stain many compounds) strategy, 31 strains of stony coral‑derived symbiotic fungi were cultured in small‑scale fermentation with potato dextrose broth (PDB) and brown rice medium at three salinities (0.3%, 3.0%, and 10.0%), respectively. Lipopolysaccharide (LPS) ‑induced BV‑2 microglia were used as the inflammation model, from which the strains with anti‑inflammatory activity were selected. Via thin‑layer chromatography (TLC),ultra‑performance liquid chromatography quadrupole time‑of‑flight mass spectrometry (UPLC‑QTOF‑MS) and feature‑based molecular networking (FBMN), the chemical diversity of the secondary metabolites from the anti‑inflammatory active strains was further analyzed. The results showed that five strains demonstrated significant anti‑inflammatory activity under the brown rice medium conditions and showed moderate or strong inhibitory activity against LPS‑ induced nitric oxide (NO) release in BV‑2 cells, with a significant concentration‑dose dependence. While the crude extract of strain C3‑9 also showed the significant inhibition to NO production at low concentrations of 10 and 20 μg/mL, further TLC and FBMN analyses revealed that the secondary metabolites of strain C3‑9 were rich in anti‑inflammatory compounds and highly diverse. The secondary metabolites of the stony coral co‑epiphytic fungus C3‑9 have significant anti‑inflammatory activity and diversity, providing a basis for the subsequent discovery of anti‑ inflammatory active compounds. [ABSTRACT FROM AUTHOR]