一株异养硝化 - 好氧反硝化细菌的筛选及氮转化特性 研究.

The excessive nitrogen in aquaculture easily causes the deterioration of water quality and threatens the growth and reproduction of aquatic animals, thus a safe and efficient nitrogen removal method is urgently needed. An ammonia‑degrading bacterium was selected from the sludge at the bottom of an aquaculture pond in Fujian province. After the strain identification, the adaptive domestication was carried out. The effects of different conditions on the growth of the strain and its ammonia‑nitrogen degradation were studied. Subsequently, nitrite was used as the only nitrogen source to detect the aerobic denitrification characteristics of the strains. The strain was identified as Bacillus aryabhattai by 16S rRNA and named as JN01. After acclimation, the growth of the strain and removal rate of ammonia nitrogen increased at varied degrees, and the removal rate of nitrogen was up to 87.29% with the initial NH4 + ‑N concentration was 200 mg/L. The results of single factor experiment showed that the degradation rate of ammonia nitrogen of the strain reached 92.78% when NH4 + ‑N concentration was 200 mg/L, sodium succinate as carbon source, carbon to nitrogen ratio was 15∶1, pH 7.5 and culture temperature was 30℃ . Under the condition that nitrite nitrogen was the only nitrogen source, strain JN01 also performed aerobic denitrification, and the degradation rate of NO2 - ‑N was 82.30%. In conclusion, B. aryabhattai JN01 has promising heterotrophic nitrification and aerobic denitrification characteristics, and has the potential to concurrently solve the excessive ammonia nitrogen and nitrite in aquaculture wastewater [ABSTRACT FROM AUTHOR]