Back to Search
Start Over
Genome-Based in silico Evaluation of the Biosafety and Probiotic Traits of Limosilactobacillus fermentum J23 Isolated from Artisanal Mexican Cheese.
- Source :
-
Food Biotechnology . 2024, Vol. 38 Issue 1, p1-29. 29p. - Publication Year :
- 2024
-
Abstract
- Comprehensive in silico analysis aimed to identify the genes involved in the biosafety and probiotic features of Limosilactobacillus fermentum J23 was performed. The strain had a single circular chromosome of 2,004,882 base pairs (bp), with a Guanine + Cytosine (G + C) content of 51.74%. The chromosome contained 2,001 genes predicted as protein-coding sequences (CDS), 86 pseudogenes, 56 tRNAs, five rRNAs, and three ncRNAs. The in silico analysis demonstrated the absence of risk-related genes corresponding to transferable/acquirable antibiotic resistance, virulence factors, toxic metabolites, and detrimental metabolic/enzymatic activities. The strain was predicted as nonpathogenic to humans and the presence of a variety of genes potentially related to its probiotic profile were revealed, including those of carbohydrate metabolism and transport, adhesion and aggregation ability, stress/adaptive response, CRISPR/Cas adaptive immunity, and immunomodulation. These findings highlight the probiotic potential of L. fermentum J23 and provide genomic clues of its biosafety to advance its future applications. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08905436
- Volume :
- 38
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Food Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 175196338
- Full Text :
- https://doi.org/10.1080/08905436.2024.2305959