Study on simultaneous determination of six kinds of growth promoting antibacterial drugs in premixes by ultra-high performance liquid chromatography.

The study was to establish a method for the simultaneous determination of six growth promoting antibacterial agents in premixes by ultra-high performance liquid chromatography. An acetonitrile-0.25% formic acid aqueous solution (50: 50) was used as the solvent, and the samples were pretreated by ultrasonic extraction. A 0.05 mol/L ammonium formate aqueous solution (pH value adjusted to 3.8 with formic acid)-acetonitrile was used as the mobile phase, and the samples were separated by gradient elution and C18 column. The results showed that the detection limit of six antibacterial agents was 1~10 mg/g. In the range of 2~100 mg/g of the added concentration, the peak areas of the main components of the six growth promoting antibacterial agents detected by the method had a good linear relationship, and the correlation coefficient R2 was 0.999 4~1.000 0. The average recoveries of six antibacterial agents were 92.2%~104.6%. The study indicates that the proposed method is efficient, reliable and sensitive, which can be used for the detection of growth promoting antibacterial agents in premixes. [ABSTRACT FROM AUTHOR]