莲草直胸跳甲短神经肽 F 受体基因 sNPFR 的克隆及表达分析.

To explore the function and expression characteristics of the short neuropeptide receptor AhsNPFR in Agasicles hygrophila, and to lay a theoretical foundation for exploring the biological control role of Agasicles hygrophila, in this study, the AhsNPFR gene was cloned using PCR technology and analyzed with bioinformatics software. The spatiotemporal expression profiles of AhsNPFR across different developmental stages and tissues were investigated by quantitative real-time PCR(qPCR). The results showed that the complete sequence of 1 669 bp was obtained, which was found to contain a 1 257 bp open reading frame(ORF), encoding 418 amino acids. The predicted molecular weight was 48.11 ku and the theoretical isoelectric point(pI) was 8.21. AhsNPFR had 7 typical conserved transmembrane domains and belonged to the GPCRs family. Phylogenetic tree analysis showed that AhsNPFR was closely related to Diabrotica virgifera virgifera sNPFR. AhsNPFR gene was expressed throughout the developmental stages tested with the highest expression levels at the first instar larva which was 9.06 times higher than those eggs, the stage with the lowest expression level. The expression level in female adult was significantly higher than that in male adult. The AhsNPFR gene was expressed in different tissues and was significantly highly expressed in the hindgut of the 3rd instar larvae, which was 12.21 times higher than those fat body. The AhsNPFR gene was significantly highly expressed in the hindgut of male and female adults, with no difference expression between the sexes across all tissues. [ABSTRACT FROM AUTHOR]