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Cross-pathogenicity of Phytophthora palmivora associated with bud rot disease of oil palm and development of biomarkers for detection.
- Source :
-
World Journal of Microbiology & Biotechnology . Feb2024, Vol. 40 Issue 2, p1-20. 20p. - Publication Year :
- 2024
-
Abstract
- Phytophthora palmivora has caused disease in many crops including oil palm in the South America region. The pathogen has had a significant economic impact on oil palm cultivation in Colombia, and therefore poses a threat to oil palm cultivation in other regions of the World, especially in Southeast Asia, the largest producer of the crop. This study aimed to look at the ability of isolates from Malaysia, Colombia, and other regions to cross-infect Malaysian oil palm, durian, and cocoa and to develop specific biomarkers and assays for identification, detection, and diagnosis of P. palmivora as a key component for the oil palm biosecurity continuum in order to contain the disease especially at the ports of entry. We have developed specific molecular biomarkers to identify and detect Phytophthora palmivora using polymerase chain reaction (PCR) and real-time loop mediated isothermal amplification (rt-LAMP) in various sample types such as soil and plants. The limit of detection (DNA template, pure culture assay) for the PCR assay is 5.94 × 10ā2 ng µlā1 and for rt-LAMP is 9.28 × 10ā4 ng µlā1. Diagnosis using rt-LAMP can be achieved within 30 min of incubation. In addition, PCR primer pair AV3F/AV3R developed successfully distinguished the Colombian and Malaysian P. palmivora isolates. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09593993
- Volume :
- 40
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- World Journal of Microbiology & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 175162796
- Full Text :
- https://doi.org/10.1007/s11274-023-03860-5